ABSTRACT
Abstract The aim of this study was to characterize the salivary metabolomic profile in adolescents with juvenile systemic lupus erythematosus (jSLE). A total of 24 adolescents with jSLE (15.92 ± 2.06 years) and 12 systemically healthy controls (15.25 ± 2.7 years) were included in the study. Participants underwent rheumatologic testing and periodontal examination, with the recording of plaque index (PI), probing depth (PD), clinical attachment level (CAL), and bleeding on probing index (BPI). Unstimulated whole saliva was collected from both groups and stored at -80 ºC. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a spectrometer operating at 500 MHz. Partial least squared discriminant analysis (PLS-DA) and orthogonal PLS-DA (O-PLS-DA) were used for statistical analysis. Mean CAL and PI were significantly increased in the group with jSLE (p < 0.01). Patients with jSLE presented a significantly different salivary metabolic profile (accuracy = 0.54; R2 = 0.86; Q2 = -0.293), significantly higher salivary levels of N-acetyl sugars, and significantly reduced levels of phenylalanine, glycine, taurine, hydroxybutyrate, and valerate compared with healthy controls (p < 0.05). It is suggested that the salivary metabolomic profile analyzed by 1H NMR in patients with jSLE presents a different fingerprint that the systemically healthy subjects. Integrating the variation of metabolites with the identification of the metabolic pathways involved seems to provide a better understanding of the influence of systemic disease on salivary metabolites.
ABSTRACT
O Lúpus Eritematoso Sistêmico (LES) é uma desordem reumática, crônica-inflamatória sistêmica e autoimune, mediada pela forte resposta anticorpo-antígeno, sob diversas formas de manifestação. Sua incidência e prevalência têm crescido mundialmente, sendo assim uma doença que ganhou destaque mais recentemente no meio científico. Por outro lado, a doença periodontal é também uma condição inflamatória, porém relacionada principalmente a uma disbiose microbiótica. Objetivo: O objetivo deste trabalho foi realizar uma revisão de literatura sobre a associação entre LES e as doenças periodontais. Materiais e Métodos: Foi realizada uma busca bibliográfica no site do pubmed, associando os termos "systemic lupus erythematosus" e "periodontal disease", sendo encontrados 104 artigos. Um artigo era incluído neste estudo se fosse publicado em português ou em inglês, analisando o LES e as doenças periodontais em seres humanos. Relatos de caso e revisões de literatura foram excluídos. Resultados: Dos 104 artigos encontrados, 24 foram selecionados pela análise do resumo e título. Dentre estes, 13 foram selecionados para serem incluídos na revisão de literatura, onde 10 estudos eram transversais, 2 eram observacionais e apenas 1 era longitudinal. Com isso, 12 dos artigos evidenciaram uma associação positiva entre o LES e as doenças periodontais. Conclusão: Na maioria dos estudos foi observada a associação do LES com as doenças periodontais (AU)
Systemic lupus erythematosus (SLE) is a rheumatic, chronic-inflammatory systemic and autoimmune disorder, mediated by the strong antibody-antigen response, under various forms of manifestation. It's incidence and prevalence have grown worldwide, therefore this disease has gained prominence more recently in the scientific environment. On the other hand, periodontal disease is also an inflammatory condition, but mainly related to microbiotic dysbiosis. Objective: The objective of this study was to review the literature on the association between SLE and periodontal diseases. Methods: A bibliographic search was performed on the pubmed site, associating the terms "systemic lupus erythematosus" and "periodontal disease", with 104 articles being found. An article was included in this study when it were published in Portuguese or in English, analyzing SLE and periodontal diseases in humans. Articles that were published in a different language than Portuguese or English, case reports and literature reviews. Results: Of the 104 articles found, 24 were selected by analysis of the abstract and title. Of these, 13 were selected to be included in the literature review, where 10 studies were cross-sectional, 2 were observational and only 1 were longitudinal. Thus, 12 of the articles showed a positive association between SLE and periodontal diseases. Conclusion: In the majority of studies, the association of SLE with periodontal diseases was observed. (AU)